FastQCFastQC Report

Summary

[OK]Basic Statistics

MeasureValue
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82641374
Sequences flagged as poor quality0
Sequence length126
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGTAT1795366621.724791264966143TruSeq Adapter, Index 8 (97% over 36bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGTATG30320643.6689419031198587TruSeq Adapter, Index 8 (97% over 35bp)
GTTCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGTAT1616270.19557637074136738TruSeq Adapter, Index 3 (97% over 34bp)
AATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGTAT1174320.14209831506431633TruSeq Adapter, Index 3 (97% over 34bp)
ATCGGAAGAGCACACGTCTGAACTCCCGTCACTCTCGCGCATCTCGTATG980360.11862823093914193TruSeq Adapter, Index 3 (96% over 33bp)
GATAGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGTAT829610.10038676268862615TruSeq Adapter, Index 3 (97% over 34bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)